It is not spoken so much about Iodine in the world. But lode, in the nature represented like iodine is as important for our body as every single needed element. In my work, I was determined iodine anion in mineral water. There are not some exact value for available quantity in mineral water and many of producers does not mentioned quantity of iodine, which is contained in each mineral water.There are not any qualified method to determine iodine in water. In old database I found out some old spectrophotometry method and I started with modifying this method according necessary parameters.After this process we determined iodine in water. According our results and qualified results our method was correct. The found results may be dangerous to people with Thyroid gland problems.